Hands-On human induced Pluripotent Stem Cell (hiPSC) Culture Training
The Stem Cell Research and Technology Resource Center offers hands-on training in the culture of human iPSCs for Basic 补苍诲听Translational Research.听
The course includes a hands-on iPSC culture workshop suitable for those with prior experience in mammalian cell culture or no prior cell culture experience. The workshop aims to quickly give you the skills and knowledge to start independently and confidently growing your iPSCs and undertaking iPSC-based experiments. Topics include:
鈥⑻齀solation and reprogramming of iPSC from PBCs and Fibroblast,
*听听听 听 听 听 Transfection systems.
鈥⑻鼵ell engineering and gene transfer, CRISPR.
鈥⑻齅aintenance of human iPSCs (feeder-dependent 补苍诲听feeders-free conditions).
鈥⑻鼸xpansion (enzymatic, non-enzymatic solutions, and manual techniques) of iPSC听(feeder-dependent 补苍诲听feeders-free conditions).
*听 听 听 听 听A next-generation PSC suspension culture system for optimal expansion and scale-up!
鈥⑻鼴anking (feeder-dependent 补苍诲听feeders-free conditions).
鈥⑻鼵ryopreservation and thawing of iPSCs听(feeder-dependent 补苍诲听feeders-free conditions).
*听听听 听 听 听 Characterization and release testing.
*听 听 听 听 听 Mycoplasma testing
*听 听 听 听 听Cell treatments
鈥⑻齈reparation of common feeder-free cell culture substrates Matrigel, Geltrex, CELLStart, Vitronectin, etc.
鈥⑻齈reparation of standard human iPSC media (feeder-dependent 补苍诲听feeders-free conditions).
鈥� 听 听 听 听 听Resource听to purchase iPSCs lines and request services.
鈥⑻齋terile technique, preventing contamination.
鈥⑻� 听 听 听 听 Differentiation of iPSCs听to NPCs, Neurons, Cardiomyocytes, Hepatocytes, and available kits. Including derivation, culturing, expansion, freezing, thawing, 补苍诲听characterization.
鈥⑻�听听听3顿听辞谤驳补苍辞颈诲: derivation, culturing, expansion, freezing, thawing, 补苍诲听characterization.
鈥⑻�And more!听听听听
贵辞谤听questions about training听and user fees, please听contact the Center.
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