Publications

  • 131) Dohrmann, P.R., Yuan, Q, , Sutton, M.D., and McHenry, C.S. (2016) DNA Polymerase III, but not Polymerase IV, Must be Bound to tau-Containing DnaX Complex to Enable Exchange into Progressing Replication Forks. J. Biol. Chem. 291, 11727-11735.
  • 130) Dohrmann, P.R., Correa, R., Frisch R.L., Rosenberg, S.M., McHenry, C.S. (2016) “The DNA polymerase III holoenzyme contains gamma and is not a trimeric polymerase.” Nucl. Acids Res. 44, 1285-1297.
  • 129) Lindow, J.C., Dohrmann, P.R., McHenry, C.S. (2015) “DNA Polymerase alpha Subunit Residues and Interactions Required for Efficient Initiation Complex Formation Identified by a Genetic Screen”J. Biol. Chem. 290, 16851-16860J. Biol. Chem.290, 16851-16860.
  • 128) Manhart, C.M., McHenry, C.S. (2015) “Identification of Subunit Binding Positions on a Model Fork and Displacements that Occur During Sequential Assembly of the E. coli Primosome.”J. Biol. Chem.290, 10828-10839.
  • 127) McHenry, C.S. (2014)“Chemical biology of DNA replication.” In: Bell E, Bond J, Klinman J, Masters B, Wells R (eds.)Molecular life sciences:an encyclopedic reference.Springer Science+Business Media New York. DOI: 10.1007/978-1-4614-6436-5_133-1.
  • 126) McHenry, C.S. (2014) “Many Bacteria use a Special Mutagenic Pol III in Place of Pol V.” In: Bell E, Bond J, Klinman J, Masters B, Wells R (eds.)Molecular life sciences: an encyclopedic reference.Springer Science+Business Media New York. DOI: 10.1007/978-1-4614-6436-5_124-1.
  • 125) McHenry, C.S. (2014) “DnaX Complex Composition and Assembly within Cells.” In: Bell E, Bond J, Klinman J, Masters B, Wells R (eds.)Molecular life sciences: an encyclopedic reference.Springer Science+Business Media New York. DOI: 10.1007/978-1-4614-6436-5_123-1.
  • 124) McHenry, C.S. (2014)“Cycling of the Lagging Strand Replicase During Okazaki Fragment Synthesis.” In: Bell E, Bond J, Klinman J, Masters B, Wells R (eds.)Molecular life sciences: an encyclopedic reference.Springer Science+Business Media New York. DOI: 10.1007/978-1-4614-6436-5_132-1
  • 123) McHenry, C.S. (2014) “DNA Polymerase III Structure.” In: Bell E, Bond J, Klinman J, Masters B, Wells R (eds.)Molecular life sciences: an encyclopedic reference.Springer Science+Business Media New York. DOI: 10.1007/978-1-4614-6436-5_131-1.
  • 122) McHenry, C.S. (2014)“Mechanism of Initiation Complex Formation.” In: Bell E, Bond J, Klinman J, Masters B, Wells R (eds.) Molecular life sciences: an encyclopedic reference.Springer Science+Business Media New York. DOI: 10.1007/978-1-4614-6436-5_128-1.
  • 121) Yuan, Q. and McHenry, C. (2014) Cycling of theE. colilagging strand polymerase is triggered exclusively by the availability of a new primer at the replication fork.Nucleic Acids Res.42, 1747-1756.
  • 120) Antony, E., Weiland, E., Yuan, Q., Manhart, C.M., Nguyen, B., Kozlov, A.G., McHenry, C. and Lohmman, T.M.(2013)Multiple C-Terminal Tails within a SingleE. coliSSB Homotetramer Coordinate DNAReplication and Repair.J. Mol. Biol.425, 4802-4819.
  • 119) Ungermannova D., Lee J., Zhang G., Dallmann H.G., McHenry C. and Liu X. (2013)High-Throughput Screening Alpha Screen Assay for Identification of Small-Molecule Inhibitors of Ubiquitin E3 Ligase SCFSkp2-Cks1.J. Biomol Screen.,8, 910-20.
  • 118) Seco, E., Zinder, J., Manhart, C.M., Piano, A.L., McHenry, C. andAyora, S. (2013) Bacteriophage SPP1in vitroDNA Replication Strategies Promote Viral and Disable Host Replication.Nucleic Acid Res.,41, 1711-1721.
  • 117) Manhart, C.M. andMcHenry, C. (2013) The PriA Replication Restart Protein Blocks Replicase Access Prior to Helicase Assembly and Directs Template Specificity through its ATPase Activity.J. Biol. Chem.,288,3989-3999.
  • 116) McHenry, C. Bacterial DNA Replicases. E. Bell, J. Bond, J. Klinman, B. Siler Masters, and R. Wells (Eds.),Molecular Life Sciences: An Encyclopedic Reference.SpringerScience+Business Media, New York.DOI:10.1007/978-1-4614-6436-5_54-2.
  • 115) Dohrmann, P.R., Manhart, C.M., Downey, C.D. andMcHenry, C. (2011) The Rate of Polymerase Release upon Filling the Gap between Okazaki Fragments is Inadequate to Support Cycling During Lagging Strand Synthesis. J. Mol. Biol.,414,15-27.
  • 114) McHenry, C. (2011) Bacterial Replicases and Related Polymerases. Curr.Opin.Chem. Biol.,15, 587-594.
  • 113) Downey, C.D., Crooke, E. andMcHenry, C. (2011) Polymerase Chaperoning and Multiple ATPase Sites Enable theE. coliDNA Polymerase III Holoenzyme to Rapidly Form Initiation Complexes. J. Mol. Biol.,412,340-353.
  • 112) McHenry, C. (2011)Breaking the Rules: Bacteria That Use Several DNA Polymerase IIIs.EMBORep.12, 408-414.
  • 111) McHenry, C. (2011) DNA Replicases from a Bacterial Perspective.Annu. Rev. Biochem.,80, 403-436.
  • 110) Wieczorek, .A,Downey, C.D., Dallmann, H.G. andMcHenry, C. (2010) Only one ATP-binding DnaX Subunit is Required for Initiation Complex Formation by theEscherichia coliDNA Polymerase III Holoenzyme. J. Biol. Chem.,285, 29049-29053.
  • 109) Dallmann, H.G.,Fackelmayer, O.J., Tomer, G., Chen, J.Y., Wiktor-Becker, A., Ferrara, T., Pope, C., Oliveira, M.T., Burgers, P.M.J., Kaguni, L.K. and McHenry, C. (2010) Parallel Multiplicative Target Screening Against Divergent Bacterial Replicases: Identification of Specific Inhibitors with Broad Spectrum Potential.Biochemistry,49, 2551-2562.
  • 108) Downey, C.D. and McHenry, C. (2010) Chaperoning of a Replicative Polymerase onto a Newly-Assembled DNA Bound Sliding Clamp by the Clamp Loader.Mol. Cell,37,481-491.
  • 107) Sanders, G.M., Dallmann, H.G. and McHenry, C. (2010) Reconstitution of theB. subtilisReplisome with 13 Proteins including Two Distinct Replicases. Mol. Cell,37, 273-281.
  • 106) Yuan,Q. and McHenry, C. (2009) Strand Displacement by DNA Polymerase III Occurs through atau-psi-chiLink to SSB Coating the Lagging Strand Template. J. Biol. Chem.,284, 31672-31679.
  • 105) Guiles, J., Sun, X., Critchley, I.A., Ochsner, U., Tregay, M., Stone, K., Bertino, J., Green, L., Sabin, R., Dean, F., Dallmann, H.G., McHenry, C. andJanic, N. (2009) Quinazolin-2-ylamino-quinazolin-4-ols as Novel Non-nucleoside Inhibitors of Bacterial DNA Polymerase III.Bioorg Med Chem Lett.,19, 800-802.
  • 104) Stano, N., Chen, J. and McHenry, C. (2006) A Coproofreading Zn+2-dependent Exonuclease within a Bacterial Replicase.Nat. Struct. Mol. Biol.,13, 458-459.
  • 103) Wieczorek, A. and McHenry, C. (2006)The N-terminal php Domain of the alpha Subunit of theE. coliReplicase Binds the epsilon Proofreading Subunit.J. Biol. Chem.,281,12561-12567.
  • 102) Jarvis, T.C., Beaudry, A.A., Bullard, J.M., Ochsner, U., Dallmann, H.G. andMcHenry, C., (2005)Discovery and Characterization of the Cryptic gamma Subunit of the Pseudomonad DNA Replicase. J. Biol. Chem.,280, 40465-40473.
  • 101) Dohrmann, P.R. andMcHenry, C., (2005) A Bipartite Polymerase–Processivity Factor Interaction: Only the Internal beta Binding Site of the alpha Subunit is Required for Processive Replication by the DNA Polymerase III Holoenzyme.J. Mol. Biol.,350, 228-239.
  • 100) Jarvis, T.C., Beaudry, A.A., Bullard, J.M., Janjic, N. and McHenry, C. (2005) Reconstitution of a minimal DNA Replicase fromPseudomonas aeruginosaand Stimulation by Non-cognate Auxiliary Factors.J. Biol Chem.,280, 7890-7900.
  • 99) McHenry, C. (2003) Chromosomal Replicases as Asymmetric Dimers: Studies of Subunit Arrangement and Functional Consequences.Mol. Microbiol.,49, 1157-1165.
  • 98) Bullard J.M., Williams J.C., Acker W.K., Jacobi C,. Janjic N. and McHenry C. (2002) DNA Polymerase III Holoenzyme fromThermus thermophilus: Identification, Expression, Purification of Components and Use to Reconstitute a Processive Replicase.J. Biol. Chem.,277, 13401-13408.
  • 97) Bullard, J., Pritchard, A.,Song, M.-S., Glover, B., Wieczorek, A., Chen, J., Janjic, N. and McHenry, C. (2002) A Three Domain Structure for the delta Subunit of the DNA Polymerase III Holoenzyme deltaDomain III Binds delta'and Assembles into the DnaX Complex. J. Biol. Chem.,277, 13246-13256.
  • 96) Muthuswami, R., Chen, J., Burnett, B., Thimmig, R., Janjic, N. and McHenry, C. (2002) The HIV Plus-Strand Transfer Reaction: Determination of Replication-Competent Intermediates and Identification of a Novel Lentiviral Element, the Primer Over-Extension Sequence.J. Mol. Biol.,315, 311-323.
  • 95) Song, M-S. and McHenry, C. (2001) Carboxyl-terminal Domain III of the delta'Subunit of DNA Polymerase III Holoenzyme Binds DnaX and Supports Cooperative DnaX-Complex Assembly. J. Biol. Chem.,276, 48709-48715.
  • 94) Duan, C., Poticha, D., Stoeckli, T.C., Petropoulos, C.J., Whitcomb, J.M., McHenry, C. and Kuritzkes, D.R. (2001) Inhibition of Purified Recombinant Reverse Transcriptase from Wild-Type and Zidovudine-Resistant Clinical Isolates of Human Immunodeficiency Virus Type 1 by Zidovudine, Stavudine, and Lamivudine Triphosphates.J. Infect. Dis.,184, 1336-1340.
  • 93) Song, M-S, Dallmann, H.G. and McHenry, C. (2001) Carboxyl-terminal Domain III of the delta' Subunit of the DNA Polymerase III Holoenzyme Binds delta.J. Biol. Chem.,276, 40668-40679.
  • 92) Glover, B., Pritchard, A. and McHenry, C. (2001) TauBinds and OrganizesEscherichia coliReplication Proteins through Distinct Domains: Domain III, shared by gammaand tau,oligomerizes DnaX. J. Biol. Chem.,276, 35842-35846.
  • 91) Pritchard, A.E. and McHenry, C. (2001) Assembly of DNA Polymerase III Holoenzyme: Co-assembly of gammaand tauis Inhibited by DnaX Complex Accessory Proteins but Stimulated by DNA polymerase III Core. J. Biol. Chem.,276, 35217-35222.
  • 90) Song, M-S, Pham, P., Olson, M., Carter, J., Franden, M., Schaaper, R. and McHenry, C. (2001) The deltaand delta'Subunits of the DNA Polymerase III Holoenzyme Are Essential for Initiation Complex Formation and Processive Elongation. J. Biol. Chem.,276, 35165-35175.
  • 89) Glover, B. and McHenry, C. (2001) The DNA Polymerase III Holoenzyme: An Asymmetric Dimeric Replicative Complex with Leading and Lagging Strand Polymerases. Cell,105, 925–934.
  • 88) Gao, D. and McHenry, C. (2001) TauBinds and OrganizesEscherichia coliReplication Proteins through Distinct Domains:Domain III, shared by gamma and tau,Binds delta-delta'and chi-psi. J. Biol. Chem.,276, 4447-4453.
  • 87) Gao, D. and McHenry, C. (2001) TauBinds and OrganizesEscherichia coliReplication Proteins through Distinct Domains: DomainIV, Located within the UniqueCarboxyl-terminus of tau,Binds theReplicationForkHelicase, DnaB.J. Biol. Chem.,276, 4441-4446.
  • 86) Gao, D. and McHenry, C. (2001) TauBinds and OrganizesE. coliReplication Proteins through Distinct Domains: Partial Proteolysis of Terminally Tagged tau to Determine Candidate Domains and to Assign Domain V as the alphaBinding Domain. J. Biol. Chem.,276, 4433-4440.
  • 85) Pritchard, A., Dallmann, G., Glover, B. and McHenry, C. (2000) A Novel Assembly Mechanism for the DNA Polymerase III Holoenzyme DnaX Complex: Association of delta-delta'with DnaX4Forms DnaX3delta-delta'.EMBO J.,19, 6536-6545.
  • 84) Dallmann, H.G., Kim, S., Pritchard, A.E., Marians, K.J. and McHenry, C. (2000) Characterization of the Unique C-terminus of theE. colitau DnaX Protein: Monomeric C-taubinds alphaand DnaB and can Partially Replace tauin Reconstituted Replication Forks.J. Biol. Chem.,275, 15512-15519.
  • 83) Glover, B.P. and McHenry, C. (2000) The DnaX-binding Subunits delta'and psiAre Bound to gammaandNot tauin the DNA Polymerase III Holoenzyme.J. Biol. Chem.,275, 3017-3020.
  • 82) Pham, P.T., Olson, M.W., McHenry, C. and Schaaper, R.M. (1999) Mismatch Extension byEscherichia coliDNA Polymerase III Holoenzyme.J. Biol. Chem.,274, 3705-3710.
  • 81) Pritchard, A.E. and McHenry, C. (1999) Identification of the Acidic Residues in the Active Site of DNA Polymerase III.J. Mol. Biol.,285, 1067-1080.
  • 80) Glover, B.P. and McHenry, C. (1998) The chi-psiSubunits of DNA Polymerase III Holoenzyme Bind to Single-stranded DNA-binding Protein (SSB) and Facilitate Replication of an SSB-coated Template.J. Biol. Chem.,273, 23476-23484.
  • 79) Pham, P.T., Olson, M.W., McHenry, C. and Schaaper, R.M. (1998) The Base Substitution and Frameshift Fidelity ofEscherichia coliDNA Polymerase III Holoenzymein Vitro.J. Biol. Chem.,273, 23575-23584.
  • 78) Marians, K.J., Hiasa, H., Kim, D.R. and McHenry, C. (1998) Role of the Core DNA Polymerase III Subunits at the Replication Fork: alphaIs the only Subunit Required for Processive Replication.J. Biol. Chem.,273, 2452-2457.
  • 77) Kim, D. R., Pritchard, A.E. and McHenry, C. (1997) Localization of the Active Site of the alphaSubunit of theEscherichia coliDNA Polymerase III Holoenzyme.J. Bacteriol.,179, 6721-6728.
  • 76) McHenry, C., Seville, M. and Cull, M.G. (1997) A DNA Polymerase III Holoenzyme-like Subassembly from an Extreme Thermophilic Eubacterium.J. Mol. Biol.,272, 178-189.
  • 75) Burnett, B. and McHenry, C. (1997) Posttranscriptional Modification of Retroviral Primers is Required for Late Stages of DNA Replication.Proc. Natl. Acad. Sci., USA94, 7210-7215.
  • 74) Seville, M., West, A., Cull, M. and McHenry, C. (1996) Fluorometric Assay for DNA Polymerases and Reverse Transcriptase.BioTechniques,21, 664-672.
  • 73) Kim, S., Dallmann, H.G., McHenry, C. and Marians, K.J. (1996) TauCouples the Leading- and Lagging-strand Polymerases at theEscherichia coliDNA Replication Fork.J. Biol. Chem.,271, 21406-21412.
  • 72) Kim, D.R. and McHenry, C. (1996) Identification of the beta-Binding Domain of the alphaSubunit ofEscherichia coliPolymerase III Holoenzyme.J. Biol. Chem.,271,20699-20704.
  • 71) Kim, D.R. and McHenry,C. (1996) Biotin Tagging Deletion Analysis of Domain Limits Involved in Protein-Macromolecular Interactions: Mapping the tau Binding Domain of the DNA Polymerase III alphaSubunit.J. Biol. Chem.,271,20690-20698.
  • 70) Kim, D.R. and McHenry, C. (1996)In VivoAssembly of Overproduced DNA Polymerase III: Overproduction, Purification and Characterization of the alpha, alpha-epsilonand alpha-epsilon-thetaSubunits.J. Biol. Chem.,271,20681-20689.
  • 69) Kim, S., Dallmann, H.G., McHenry, C. and Marians, K. J. (1996) TauProtects betain the Leading-strand Polymerase Complex at the Replication Fork.J. Biol. Chem.,271, 4315-4318.
  • 68) Pritchard, A.E., Dallmann, H.G. and McHenry, C. (1996)In VivoAssembly of the tau-Complex of the DNA Polymerase III Holoenzyme Expressed from a Five-Gene Artificial Operon: Cleavage of the tau-complex to Form a Mixed tau-gammacomplex by the OmpT Protease.J. Biol. Chem.,271, 10291-10298.
  • 67) Kim, S., Dallmann, H.G., McHenry, C. and Marians, K.J. (1996) Coupling of a Replicative Polymerase and Helicase: A tau-DnaB Interaction Mediates Rapid Replication Fork Movement.Cell,84, 643-650.
  • 66) Olson, M., Dallmann, H.G. and McHenry, C. (1995) DnaX-Complex ofE. coliDNA Polymerase III Holoenzyme: The chi-psiComplex Functions by Increasing the Affinity of tauand gammafor delta-delta'to a Physiologically Relevant Range.J. Biol. Chem.,270, 29570-29577.
  • 65) Dallmann, H.G. and McHenry, C. (1995) DnaX-Complex ofEscherichia coliDNA Polymerase III Holoenzyme: Physical Characterization of the DnaX Subunits and Complexes.J. Biol. Chem.,270, 29563-29569.
  • 64) Dallmann, H.G., Thimmig, R. and McHenry, C. (1995) DnaX-Complex ofE. coliDNA Polymerase III Holoenzyme: Central Role of tauin Initiation Complex Assembly and in Determining the Functional Asymmetry of Holoenzyme.J. Biol. Chem.,270, 29555-29562.
  • 63) Cull, M. and McHenry, C. (1995) Purification ofE. coliDNA Polymerase III Holoenzyme, inDNA ReplicationMethods in Enzymology, Vol. 262, Chapter 3 (Campbell, J., volume ed.), pp 22-35, Academic Press, New York.
  • 62) Reems, J., Wood, S. and McHenry, C. (1995)E. coliDNA Polymerase III Holoenzyme Subunits alpha, beta and gammaDirectly Contact the Primer Template.J. Biol Chem.,270, 5606-5613.
  • 61) Reems, J. and McHenry, C. (1994)E. coliDNA Polymerase III Holoenzyme Footprints Three Helical Turns of Its Primer.J. Biol. Chem.,269, 33091-33096.
  • 60) You, J-C. and McHenry, C. (1994) HIV Nucleocapsid Protein Accelerates Strand Transfer of the Terminally Redundant Sequences Involved in Reverse Transcription.J. Biol. Chem.,269, 31491-31495.
  • 59) Hottiger, M., Podust, V., Thimmig, R., McHenry, C. and Hübscher, U. (1994) Strand Displacement Activity of the Human Immunodeficiency Virus Type 1 Reverse Transcriptase Heterodimer and Its Individual Subunits.J. Biol. Chem.,269, 986-991.
  • 58) Carter, J., Franden, M., Lippincott, J. and McHenry, C. (1993) Identification, Molecular Cloning and Characterization of the Gene Encoding the chi Subunit of DNA Polymerase III Holoenzyme ofEscherichia coli.Mol. Gen. Genet.,241, 399-408.
  • 57) Carter, J., Franden, M., Aebersold, R. and McHenry, C. (1993) Identification, Isolation, and Overexpression of the Gene Encoding the psiSubunit of DNA Polymerase III Holoenzyme.J. Bacteriol.,175, 5604-5610.
  • 56) Thimmig, R. and McHenry, C. (1993) HIV Reverse Transcriptase: Expression inE. coli, Purification and Characterization of a Functionally and Structurally Asymmetric Dimeric Polymerase.J. Biol. Chem.,268, 16528-16536.
  • 55) You, J-C. and McHenry, C. (1993) HIV Nucleocapsid Protein: Expression inE. coli, Purification and Characterization.J. Biol. Chem.,268, 16519-16527.
  • 54) Carter, J., Franden, M., Aebersold, R., Kim, D-R. and McHenry, C. (1993) Isolation, Sequencing and Overexpression of the Gene Encoding the theta Subunit of DNA Polymerase III Holoenzyme.Nucleic Acids Res.,21, 3281-3286.
  • 53) Carter, J., Franden, M., Aebersold, R. and McHenry, C. (1993) Identification, Isolation and Characterization of the Structural Gene Encoding the delta' Subunit ofE. coliDNA Polymerase III Holoenzyme.J. Bacteriol.,175, 3812-3822.
  • 52) Carter, J., Franden, M., Aebersold, R. and McHenry, C. (1992) Molecular Cloning, Sequencing and Overexpression of the Structural Gene Encoding the deltaSubunit ofE. coliDNA Polymerase III Holoenzyme.J. Bacteriol.,174, 7013-7025.
  • 51) Wu, C.A., Zechner, E.L., Reems, J., McHenry, C., and Marians, K.J. (1992) Coordinated Leading- and Lagging-strand Synthesis at theEscherichia coliDNA Replication Fork: V. Primase Action Regulates the Cycle of Okazaki Fragment Synthesis.J. Biol. Chem.,267, 4074-4083.
  • 50) Wu, C.A., Zechner, E.L., Hughes, A.J.,Jr., Franden, M., McHenry, C. and Marians, K.J. (1992) Coordinated Leading- and Lagging-strand Synthesis at theEscherichia coliDNA Replication Fork: IV. Reconstitution of an Asymmetric, Dimeric DNA Polymerase III Holoenzyme.J. Biol. Chem.,267, 4064-4073.
  • 49) Griep, M. A. and McHenry, C. (1992) Fluorescence Energy Transfer Between the Primer and the betaSubunit of the DNA Polymerase III Holoenzyme .J. Biol. Chem.,267, 3052-3059.
  • 48) McHenry, C. (1991) DNA Polymerase III Holoenzyme: Components, Structure and Mechanism of a True Replicative Complex.J. Biol. Chem.,266, 19127-19130.
  • 47) McHenry, C. (1992) DNA Replication (invited article) inEmerging Targets for Antibacterial and Antifungal Chemotherapy,(J. Sutcliffe and N. Georgopapadakou, eds.), pp. 37-67, Chapman & Hall, New York.
  • 46) Flower, A. and McHenry, C., (1991) Transcriptional Organization of theEscherichia coli dnaXGene.J. Mol. Biol.,220, 649-658.
  • 45) Hughes, A., Bryan, S., Chen, H. Moses, R. and McHenry, C. (1991)Escherichia coliDNA Polymerase II is Stimulated by DNA Polymerase III Holoenzyme Auxiliary Subunits.J. Biol. Chem.,266, 4568-4573.
  • 44) Reems, J., Griep, M. and McHenry, C. (1991) The Proofreading Activity of DNA Polymerase III Responds Like Elongation Activity to Auxiliary Subunits.J. Biol. Chem.,266, 4878-4882.
  • 43) Griep, M. and McHenry, C. (1990) Dissociation of the DNA Polymerase III Holoenzyme beta2Subunits is Accompanied by a Conformational Change at Distal Cysteines 333.J. Biol. Chem.,265, 20356-20363.
  • 42) Griep, M. Reems, J., Franden, M. and McHenry, C. (1990) Reduction of the Potent DNA Polymerase III Holoenzyme 3'-->5' Exonuclease Activity by Template-Primer Analogs.Biochemistry,29, 9006-9014.
  • 41) Flower, A. and McHenry, C. (1990) The gammaSubunit of DNA Polymerase III Holoenzyme ofEscherichia coliis Produced by Ribosomal Frameshifting.Proc. Natl. Acad. Sci. U.S.A.,87, 3713-3717.
  • 40) McHenry, C., Griep, M. Tomasiewicz, H. and Bradley, M. (1989) Functions and Regulation of DNA Polymerase III Holoenzyme, An Asymmetric Dimeric DNA Polymerase, inMolecular Mechanisms in DNA Replication and Recombination(C. Richardson and I.R. Lehman, eds.), pp. 115-126, Alan R. Liss, Inc., New York.
  • 39) Griep, M. and McHenry, C. (1989) Glutamate Overcomes the Salt Inhibition of DNA Polymerase III Holoenzyme.J. Biol. Chem.,264, 11294-11301.
  • 38) Cull, M. and McHenry, C. (1990) Preparation of Extracts from Prokaryotes in Guide to Protein Purification, inMethods in Enzymology,182, Chapter 12 (volume Deutscher, M., ed.), pp. 147-154, Academic Press, New York.
  • 37) McHenry, C. (1988) The Asymmetric Dimeric Polymerase Hypothesis: A Progress Report. Biochem. Biophys. Acta(invited review),951, 240-248.
  • 36) McHenry, C. (1989) Replication of the Human Immunodeficiency Virus inMolecularBiology of Chromosome Function(K. Adolph, ed.), Ch. 5, pp. 97-129, Springer-Verlag, New York.
  • 35) Franden, M. and McHenry, C. (1988) Detection of an Epitope, Not Required for Polymerization, That is Conserved betweenE.coli DNA Polymerases I and III and Bacteriophage T4 DNA Polymerase.Nucleic Acids Res.,16, 6353-6360.
  • 34) Griep M. and McHenry, C. (1988) The Dimer of the betaSubunit ofEscherichia coliDNA polymerase III Holoenzyme is Dissociated into Monomers upon Binding Magnesium(II).Biochemistry,27, 5210-5215.
  • 33) McHenry, C.,Tomasiewicz, H., Griep, M., Fürste, P. and Flower, A. (1988) DNA Polymerase III Holoenzyme: Mechanism and Regulation of a True Replicative Complex inDNA Replication and Mutagenesis(Moses, R and Summers, W., eds.), pp. 14-26, American Society for Microbiology, Washington, D.C.
  • 32) McHenry, C. (1988) DNA Polymerase III Holoenzyme ofEscherichia coli.Ann. Rev. Biochem.,57, 519- 550.
  • 31) Santi, D., McHenry, C., Raines, R. and Ivanetich, K. (1987) Kinetics and Thermodynamics of the Interaction of 5-Fluoro-2'-Deoxyuridylate with Thymidylate Synthase.Biochemistry,26, 8606-8613.
  • 30) McHenry, C., Flower, A.M. and Hawker, J.R. (1988) Coordination of Leading with Lagging Strand Synthesis by the Asymmetric Dimeric DNA Polymerase III Holoenzyme ofE. coli,inCancer Cells 6: Eukaryotic DNA Replication(Kelly, T. and Stillman, B., eds.), pp. 35-41, Cold Spring Harbor, New York.
  • 29) Hawker, J.R. and McHenry, C. (1987) Monoclonal Antibodies Specific for the tauSubunit of the DNA Polymerase III Holoenzyme ofEscherichia coli: Use to Demonstrate that tauis the Product of thednaZX Gene and that both it and gamma,thednaZ Gene Product, are Integral Components of the Same Enzyme Assembly.J. Biol. Chem.,262, 12722-12727.
  • 28) Tomasiewicz, H. and McHenry, C. (1987) Sequence Analysis of theEscherichia colidnaE Gene.J. Bacteriol.,169, 5735-5744.
  • 27) Oberfelder, R. and McHenry, C. (1987) Characterization of 2’ (3’)-Trinitrophenyl-ATP as an Inhibitor of ATP-dependent Initiation Complex Formation between the DNA Polymerase III Holoenzyme and Primed DNA.J. Biol. Chem.,262, 4190-4194.
  • 26) Flower, A. and McHenry, C. (1986) The AdjacentdnaZ anddnaX Genes ofEscherichia coliare Contained within One Continuous Open Reading Frame.Nucleic Acids Res.,14,8091-8101.
  • 25) Kwon-Shin, O., Bodner, J., McHenry, C. and Bambara, R. (1987) Properties of Initiation Complexes Formed betweenEscherichia coliDNA Polymerase III Holoenzyme and Primed DNA in the Absence of ATP.J. Biol. Chem.,262,2121-2130.
  • 24) McHenry, C., Oberfelder, R., Johanson, K., Tomasiewicz, H. and Franden, M. (1987) Structure and Mechanism of the DNA Polymerase III Holoenzyme, inDNA Replication and Recombination(Kelly, T. and McMacken, R., eds.), pp 47-61, Alan R. Liss, Inc., New York.
  • 23) Johanson, K., Haynes, T. and McHenry, C. (1986) Chemical Characterization and Purification of the betaSubunit of the DNA Polymerase III Holoenzyme from an Overproducing Strain. J. Biol. Chem.,261, 11460-11465.
  • 22) LaDuca, R., Crute, J., McHenry, C. and Bambara, R. (1986) The betaSubunit of theEscherichia coliDNA Polymerase III Holoenzyme Interacts Functionally with the Catalytic Core in the Absence of other Subunits. J. Biol. Chem.,261, 7550-7557.
  • 21) McHenry, C. (1985) DNA Polymerase III Holoenzyme ofEscherichia coli: Components and Function of a True Replicative Complex. (invited review).Mol. Cell. Biochem.,66, 71-85.
  • 20) Wu, Y.H., Franden, M., Hawker, J.R. and McHenry, C. (1984) Monoclonal Antibodies Specific for the alphaSubunit of theEscherichia coliDNA Polymerase III Holoenzyme. J. Biol. Chem.,259, 12117-12122.
  • 19) Shepard, D., Oberfelder, R. Welch, M. and McHenry, C. (1984) Determination of the Precise Location and Orientation of theEscherichia coli dnaE Gene. J. Bacteriol.,158, 455-459.
  • 18) McHenry, C. and Johanson, K. (1984) DNA Polymerase III Holoenzyme ofEscherichia coli: An Asymmetric Dimeric Replicative Complex Containing Distinguishable Leading and Lagging Strand Polymerases inProteins Involved in DNA Replication, (Hübscher, U., and Spadari, S., eds.), pp 315-319,Plenum Press.
  • 17) Johanson, K. and McHenry, C. (1984) Adenosine 5'-0-(3-thiotriphosphate) Can Support the Formation of an Initiation Complex between the DNA Polymerase III Holoenzyme and Primed DNA.J. Biol. Chem.,259, 4589-4595.
  • 16) La Duca, R., Fay, P., Chuang, C., McHenry, C. and Bambara, R. (1983) Site-Specific Pausing of DNA Synthesis Catalyzed by Four Forms ofEscherichia coliDNA Polymerase III.Biochemistry,22, 5177-5188.
  • 15) Crute, J., La Duca, R., Johanson, K., McHenry, C. and Bambara, R. (1983) Excess betaSubunit Can Bypass the ATP Requirement for Highly Processive Synthesis by theEscherichia coliDNA Polymerase III Holoenzyme.J. Biol. Chem.,258, 11344-11349.
  • 14) Welch, M. and McHenry, C. (1982) Cloning and Identification of the Product of thednaE Gene ofEscherichia coli.J. Bacteriol.,152, 351-356.
  • 13) Johanson, K. and McHenry, C. (1982) The betaSubunit of the DNA Polymerase III Holoenzyme Becomes Inaccessible to Antibody after Formation of an Initiation Complex with Primed DNA. J. Biol. Chem.,257, 12310-12315.
  • 12) Fay, P., Johanson, K., McHenry, C. and Bambara, R. (1982) Size Classes of Products Synthesized Processively by Two Subassemblies ofEscherichia coliDNA Polymerase III Holoenzyme. J. Biol. Chem.,257, 5692-5699.
  • 11) McHenry, C. (1982) Purification and Characterization ofEscherichia coliDNA Polymerase III': Identification of tauas a Subunit of the DNA Polymerase III Holoenzyme. J. Biol. Chem.,257, 2657-2663.
  • 10) Johanson, K. and McHenry, C (1981) Role of the betaSubunit of theEscherichia coliDNA Polymerase III Holoenzyme in the Initiation of DNA Elongation inStructure and DNA - Protein Interactions of Replication Origins(D. Ray, ed.), pp. 425-436, Academic Press, New York.
  • 9) McHenry, C. and Kornberg, A. (1981) DNA Polymerase III Holoenzyme, inThe Enzymes,Nucleic Acids, Vol. 14, Pt. A, Ch. 3 (P. Boyer, ed.), pp. 39-50, Academic Press, New York.
  • 8) McHenry, C. (1980) Multiple Forms of DNA Polymerase III ofEscherichia coliin:Mechanistic Studies of DNAReplication and Genetic Recombination(B. Alberts, ed.), pp. 569-577, Academic Press, New York.
  • 7) Fay, P. Johanson, K., McHenry, C. and Bambara, R. (1981) Size Classes of Products Synthesized Processively by DNA Polymerase III and DNA Polymerase III Holoenzyme ofEscherichia coli. J. Biol. Chem.,256, 976-983.
  • 6) Johanson, K. and McHenry, C. (1980) Purification and Characterization of the betasubunit of the DNA Polymerase III Holoenzyme ofEscherichia coli.J. Biol.Chem.,255, 10984-10990.
  • 5) McHenry, C. and Crow, W. (1979) DNA Polymerase IIIEscherichia coli: Purification and Identification of Subunits. J. Biol. Chem.,254, 1748-1753.
  • 4) McHenry, C. and Kornberg, A. (1977) DNA Polymerase III Holoenzyme ofEscherichia coli: Purification and Resolution into Subunits. J. Biol.Chem.,252, 6478-6484and correction of publishers inversion, left to right, of figure 3, vol 253, p 645.
  • 3) Santi, D., McHenry, C. and Sommer, H. (1974) Mechanism of Interaction of Thymidylate Synthetase with 5-Fluorodeoxyuridylate. Biochemistry,13, 471-481.
  • 2) Santi, D., McHenry, C. and Perriard, E. (1974) A Filter Assay for Thymidylate Synthetase Using 5-Fluoro-2'-deoxyuridylate as an Active Site Titrant.Biochemistry,13, 467-470.
  • 1) Santi, D. andMcHenry, C. (1972) 5-Fluorodeoxyuridylate: Covalent Complex with Thymidylate Synthetase. Proc. Nat. Acad. Sci.,69, 1855-1857.